Table 1.
E. coli strains used in this work.
| HB101 | F-, hsdS20(rB-, mB-), xyl5, l-, recA13, galK2, ara14, supE44, lacY1, rpsL20(strr), leuB6, mtl-1, thi-1 |
(AllianceBio) |
| BW25113 |
rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1 |
(6) |
| BW38985 | like BW25113, except ΔrpoS::kan | (2) |
| BW39121 | like BW25113, except Δhns::kan | (2) |
| BW39182 | like BW25113, except ΔygbF::kan | (2) |
| BW39183 | like BW25113, except ΔygbT::kan | (2) |
| BW39184 | like BW25113, except ΔygcH::kan | (2) |
| BW39185 | like BW25113, except ΔygcI::kan | (2) |
| BW39186 | like BW25113, except ΔygcJ::kan | (2) |
| BW39187 | like BW25113, except ΔygcK::kan | (2) |
| BW39188 | like BW25113, except ΔygcL::kan | (2) |
| BW39189 | like BW25113, except ΔygcB::kan | (2) |
| BW39292 | like BW25113, except ΔCRISPR-1::kan | This study |
| BW39371 | like BW25113, except ΔygbF | BW39182 KanS with pCP20a |
| BW39372 | like BW25113, except ΔygbT | BW39183 KanS with pCP20 |
| BW39373 | like BW25113, except ΔygcH | BW39184 KanS with pCP20 |
| BW39374 | like BW25113, except ΔygcI | BW39185 KanS with pCP20 |
| BW39375 | like BW25113, except ΔygcJ | BW39186 KanS with pCP20 |
| BW39376 | like BW25113, except ΔygcK | BW39187 KanS with pCP20 |
| BW39377 | like BW25113, except ΔygcL | BW39188 KanS with pCP20 |
| BW39378 | like BW25113, except ΔygcB | BW39189 KanS with pCP20 |
| BW39651 | like BW25113, but with T3 spacer | This study |
| BW39671 | like BW39651, except Δhns::kan | This study |
a
Elimination of antibiotic resistance gene was performed as described elsewhere (6)