Table 1.

Effects of ISP-1 on ethanol-induced alterations in lipid levels in P7 brains

Lipid	Vehicle	ISP-1	Ethanol	Ethanol+ISP-1
Ceramide	60.1 ± 6.1	38.7 ± 2.6	*84.7 ± 3.2	43.8 ± 2.2
TG	246 ± 9.1	256 ± 14.3	352 ± 28	319 ± 15
ChE	28 ± 6.0	20 ± 8.4	*123 ±18	76 ± 13
NAPE	16.3 ± 2.2	22.2 ± 2.5	*68.7 ± 7.1	38.3 ± 4.0
SM	444 ± 18	371 ± 45	422 ± 26	388 ± 24
GD1a	535 ± 26	474 ± 11	518 ± 8.3	467 ± 2.1

P7 mice were injected via icv with 2 μl of ISP-1 (1 μg/μl in saline containing 1% DMSO) or the vehicle. One hour after the injection, mice were injected subcutaneously twice (2 h apart) with saline or ethanol. Twenty-four hours after the first ethanol/saline injection, brains were removed for lipid analyses. Values, presented as ng/mg wet weight, are mean ± SEM (n=4).

^*The value of ethanol group is significantly (p<0.05) different from all other groups by ANOVA with Bonferroni’s post hoc test.