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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Cell Microbiol. 2010 Oct;12(10):1446–1462. doi: 10.1111/j.1462-5822.2010.01481.x

Fig. 3. Confirmation of mini-var transgene integration into the IT4var3 locus and miniPfEMP1 expression.

Fig. 3

A. PCR analysis of transfected lines was performed using the following primers: 1091 and 1094 to detect unrecombined attB sites; 1091 and 1090 to amplify the 5′ attL junction; 594 and 1094 to amplify the 3′ attR junction; and 594 and 1090 to detect unrecombined plasmid or plasmid that integrated as a concatemer. PCR product sizes are indicated. “P” indicates the attP plasmid used in the generation of each line.

B. Western blot of protein extracts from transgenic parasite lines expressing miniPfEMP1s. These extracts were probed with antibodies to GFP (top panel), or BiP (used as a loading control and shown on the bottom panel). The expected miniPfEMP1 product sizes are listed to the left of panel.