Table 1.
Potential proteotypic peptides suitable for quantification of CYP-isoforms using 1D-gel LC-MS/MS (LCQduo and Q-TOF) and 2D-LC-MS/MS (Q-TOF) formats.
| CYP- isoform |
Unique amino acid sequence1 observed from human liver microsomes by LC-MS/MS |
LCQ 1D-gel2 |
Q-TOF 1D-gel2 |
Q-TOF 2D-LC2 |
|---|---|---|---|---|
| CYP1A2 | DITGALFK | x | x | |
| FLWFLQK | x | |||
| MMLFGMGK | x | x | ||
| ASGNLIPQEK | x | |||
| IGSTPVLVLSR | x | x | x | |
| VDLTPIYGLTMK | x | x | ||
| FLTADGTAINKPLSEK | x | x | x | |
| TVQEHYQDFDKNSVR | x | |||
| TVQEHYQDFDK | x | |||
| NTHEFVETASSGNPLDFFPILR | x | |||
| GRPDLYTSTLITDGQSLTFSTDSGPVWAAR | x | |||
| CYP2A6 | GTEVYPMLGSVLR | x | x | x |
| GTGGANIDPTFFLSR | x | x | x | |
| DPSFFSNPQDFNPQHFLNEK | x | x | ||
| CYP2B6 | NLQEINAYIGHSVEK | x | ||
| IAMVDPFFR | x | |||
| CYP2C8 | SFTNFSK | x | ||
| NLNTTAVTK | x | |||
| EALIDNGEEFSGR | x | x | ||
| DQNFLTLMK | x | x | ||
| VQEEIDHVIGR | x | x | x | |
| VQEEAHCLVEELR | x | x | ||
| EHQASLDVNNPR | x | |||
| VKEHQASLDVNNPR | x | |||
| YSDLVPTGVPHAVTTDTK | x | |||
| KLPPGPTPLPIIGNMLQIDVK | x | |||
| CYP2C9 | GIFPLAER | x | ||
| GTTILISLTSVLHDNK | x | |||
| LPPGPTPLPVIGNILQIGIK | x | x | ||
| SHMPYTDAVVHEVQR | x | x | x | |
| GKLPPGPTPLPVIGNILQIGIK | x | |||
| EKHNQPSEFTIESLENTAVDLFGAGTETTSTTLR | x | |||
| CYP2E1 | EALLDYK | x | ||
| YPEIEEK | x | |||
| HEDYNDEK | x | |||
| GDLPAFHAHR | x | x | ||
| GIIFNNGPTWK | x | x | ||
| FKPEHFLNENGK | x | x | ||
| EALLDYKDEFSGR | x | x | ||
| FITLVPSNLPHEATR | x | x | x | |
| VKEHHQSLDPNCPR | x | |||
| DLTDCLLVEMEKEK | x | |||
| GTVVVPTLDSVLYDNQEFPDPEK | x | x | x | |
| EAHFLLEALR | x | x | x | |
| FGPVFTLYVGSQR | x | |||
| YSDYFKPFSTGK | x | |||
| QEMPYMDAVVHEIQR | x | |||
| DRQEMPYMDAVVHEIQR | x | |||
| CYP2J2 | VIGQGQQPSTAAR | x | ||
| FEYQDSWFQQLLK | x | |||
| CYP3A4 | EVTNFLR | x | x | |
| GVVVMIPSYALHR | x | |||
| LSLGGLLQPEKPVVLK | x | x | ||
| LGIPGPTPLPFLGNILSYHK | x | |||
| VWGFYDGQQPVLAITDPDMIK | x | x | ||
| APPTYDTVLQMEYLDMVVNETLR | x | x | ||
| CYP4A11 | VATALTLLR | x | x | x |
| QFAMNELK | x | |||
| CYP4F2 | FDPENIK | |||
| SVINASAAIAPK | x | x | ||
| HVTQDIVLPDGR | x | |||
| CYP4F3 | WQLLASEGSAR | x | ||
| CYP4F11 | TLPTQGIDDFLK | x | x | |
| CYP4F12 | LVHDFTDAVIR | x | ||
| CYP4V2 | EFFQQIIEYTEEYR | x | ||
| SVSEDCEVAGYR | x | |||
| FLEPWLGLGLLTSTGNK | x | |||
| CYP7B1 | SLDILLESMMQNLK | x | ||
| CYP8B1 | NMFEFLK | x | ||
| EEATQVLGEAR | x | |||
| AVREEATQVLGEAR | x | |||
| KFDLLFPR | x | |||
| KLDFGQYAK | x | |||
| WGFGTMQPSHDVR | x | |||
| DKEQDLLQAGELFMEFR | x | |||
| CYP20A1 | TLDPFETMLK | x | ||
| LTPVSAQFQDIEGK | x | |||
| CYP51A1 | NEDLNAEDVYSR | x | ||
| IDDILQTLLDATYK | x | |||
| TFTYLLGSDAAALLFNSK | x | |||
| SPPYIFSPIPFLGHAIAFGK | x |
1
Unique amino acid sequences were confirmed by performing a Blast Search (http://expasy.org/tools/blast/) against a human protein database. Note, peptides containing chemically reactive residues (C, M, W) and unstable sequences (N-G, N-term Q or N-term N) may not be suitable as proteotypic peptides.
2
Observation of a unique peptide indicated by x.