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. 2010 Jul 28;46(2):127–132. doi: 10.1159/000319396

Fig. 2.

Fig. 2.

Agarose gel electrophoresis of PCR amplimers. Lanes 2 and 6 were from DNA extracted directly from 1 catheter section (section 1); lanes 3 and 7 were extracted from a second section (section 2) taken from the same catheter. Lanes 2, 3, 4 and 5 are amplimers from the S. aureus hutH primer set Sau562/Sau1155; lanes 6, 7 and 8 are from the S. aureus 23S Sau327/Sau1645 primer set. Parallel black lines: expected amplicon size for the 2 primer sets. Catheter section 1 demonstrated amplimers of the expected molecular weight for both primer sets, indicating a positive identification for S. aureus. However, catheter section 2 was negative. Lanes 4 and 8 were the positive controls from DNA extracted from S. aureus ATCC No. 25923. DNA extracted from S. epidermidis ATCC No. 35984 was used as a negative control against the hutH primer set in lane 5; the absence of any amplimer demonstrates that the primer set tested is actually S. aureus-specific. Lane 1 is a ladder of molecular weight markers using the Track-It™ 1 Kb Plus DNA Ladder (Invitrogen, Carlsbad, Calif., USA).