Table 1.

HBV DNA-specific primers used in nested PCR, clone analysis, and sequencing

Primer	Nucleotide sequence (5′ → 3′)	Nucleotide position#	Polarity	Ref.
S2–1	CAAGGTATGTTGCCCGTTTG	455–474	Sense	[28]
S088	TGTTGCCCGTTTGTCCTCTA	462–471	Sense	[29]
S1–2	GCCATTTGTTCAGTGGTTCG	685–704	Antisense	[28]
S2–2	TGGCTCAGTTTACTAGTGCC	668–687	Antisense	[28]
PS8–1	GTCACCATATTCTTGGGAAC	2817–2836	Sense	[30]
HS6–2	GCCAAGTGTTTGCTGACGCA	1175–1194	Antisense
HS4–2	CCTATTGATTGGAAGGTGTG	970–989	Antisense	[30]
T728	GGAATCAAACCTTATTATCC	2688–2707	Sense	[30]
T703	CAGAGTCTAGACTCGTGGTG	242–261	Antisense	[30]
PS5–2	CTCGTGTTACAGGCGGGGTT	191–210	Antisense
M13F	GCCAGGGTTTTCCCAGTCACGAC	2949–2972	Sense
M13R	GTCATAGCTGTTTCCTGTGTGA	176–197	Antisense

Number based on EcoRI site numbering