Fig. 9.

Mean net Ca²⁺ uptake (a), titratable alkalinity uptake (b), and ammonia excretion (c) rates (μmol g⁻¹ h⁻¹ ± SEM) under control (bicarbonate-buffered, 680 μmol l⁻¹ HCO₃ ⁻) and nominally HCO₃ ⁻-free (HEPES-buffered (BFH), 7.5 μmol l⁻¹ HCO₃ ⁻, 3 mmol l⁻¹ HEPES) media conditions. Values are for day 7 egg masses that were fluxed for 24 h. N = 8 for controls and N = 7 for treatment. *Significantly different from bicarbonate-buffered (P < 0.05)