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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: J Immunol. 2009 Dec 28;184(3):1419–1424. doi: 10.4049/jimmunol.0901907

FIGURE 3.

FIGURE 3

Intracellular NA trafficking is unaffected by the SIINFEKL epitope. A, MDCK cells were infected with the indicated PR8 viruses for 30 min at 37°C and then cultured for 6 h. Cells were fixed and permeabilized with 0.5% Triton X-100, then stained with rabbit polyclonal NA Abs (green) or a mouse mAb (red) that recognizes the mature, dimerized form of NA. Nuclear staining is shown in blue. B, The GC and post-GC compartments of infected cells were identified by staining with mouse mAb H17-L2 against the IAV HA trimer (green, top). The entire secretory pathway was identified by staining cells with the HA-specific mAb Y8-10C2 (HA-All, green, bottom). Corresponding NA staining with rabbit polyclonal Abs (NA-All) is shown in red.