. Author manuscript; available in PMC: 2011 Sep 3.

Published in final edited form as: Biochem Biophys Res Commun. 2010 Aug 3;399(4):537–541. doi: 10.1016/j.bbrc.2010.07.105

Table 1.

Resting membrane potential (RMP), input resistance and current density of transfected Kir4.1 wild-type and mutants in HEK293 cells.

	number	RMP(mV)	Input Resistance(MΩ)	Current density (pA/pF, @-140mV)
untransfected	12	−36.8 ± 2.5	1908.2 ± 259.0	−1.8 ± 0.2
Kir4.1	11	−68.4 ± 0.6	24.0 ± 4.2^*	−341.5 ± 50.3^*
R65P	13	−63.3 ± 1.6	572.5 ± 165.8^*	−5.2 ± 1.0^*
A167V	14	−66.9 ± 0.9	160.5 ± 24.8^*	−23.0 ± 3.8^*
R297C	17	−34.9 ± 1.2	2777.8 ± 586.4^†	−2.4 ± 0.3^†
C140R	15	−39.0 ± 1.2	2543.0 ± 806.2^†	−2.0 ± 0.2^†
R199X	11	−35.6 ± 1.6	1548.9 ± 285.4^†	−2.5 ± 0.4^†
T164I	13	−36.0 ± 1.7	2404.2 ± 295.9^†	−3.3 ± 0.3^†
Kir4.1 +Kir4.1	12	−66.1 ± 0.6	14.0 ± 1.0	−354.2 ± 26.3
R65P+Kir4.1	08	−66.3 ± 0.3	16.3 ± 2.5^‡	−341.3 ± 30.4^‡
A167V+Kir4.1	11	−66.5 ± 0.3	15.0 ± 1.2^‡	−324.1 ± 29.3^‡
R297C+Kir4.1	10	−66.6 ± 0.3	16.2 ± 1.2^‡	−333.3 ± 32.5^‡
C140R+Kir4.1	09	−66.9 ± 0.5	13.6 ± 0.6^‡	−341.8 ± 25.0^‡
R199X+ Kir4.1	11	−67.5 ± 0.7	14.8 ± 1.1^‡	−344.4 ± 21.0^‡
T164I+ Kir4.1	11	−65.6 ± 0.5	14.2 ± 1.4^‡	−326.7 ± 16.0^‡
Kir4.1+Kir5.1	12	−66.1 ± 0.6	11.6 ± 0.7	−366.2 ± 21.4
R65P+ Kir5.1	10	−65.4 ± 0.6	94.1± 11.7^§	−51.1 ± 12.3^§
A167V+ Kir5.1	08	−66.8 ± 0.7	67.7 ± 11.6^§	−83.3 ± 9.6^§
R297C+Kir5.1	10	−39.9 ± 2.2	1624.4 ± 275.8^§	−3.7 ± 0.4^§
C140R+Kir5.1	10	−35.9 ± 1.6	2510.6 ± 551.6^§	−2.0 ± 0.4^§
R199X+ Kir5.1	08	−35.1 ± 0.7	1794.9 ± 381.8^§	−2.0 ± 0.2^§
T164I+ Kir5.1	11	−38.5 ± 1.9	1717.1 ± 158.8^§	−3.8 ± 0.4^§

represents significant values (P< 0.05), when compared to t he data obtained in untransfected group.

^†

represents no significant values (P>0.05), when compared to the data obtained in untransfected group.

^‡

represents no significant values (P>0.05), when compared to the data obtained in the wild-type group.

^§

represents no significant values (P>0.05), when compared to the data obtained in kir4.1-Kir5.1 cotransfection.