Table 3.
Examples of features of corresponding image objects (a single nucleus or a group of nuclei) that were independently extracted from images before and after straightening
| Image object | Type of information | Size | Mean intensity | Surface area | Circularity |
|---|---|---|---|---|---|
| A | Before | 8010 | 39.643 | 2138.801 | 1.161 |
| After | 8080 | 39.065 | 2179.074 | 1.184 | |
| Absolute difference | 70 | 0.579 | 40.272 | 0.023 | |
| Ratio | 0.009 | 0.015 | 0.019 | 0.019 | |
| B | Before | 13799 | 42.868 | 4018.091 | 1.736 |
| After | 13229 | 42.688 | 3908.548 | 1.737 | |
| Absolute difference | 570 | 0.180 | 109.543 | 0.001 | |
| Ratio | 0.042 | 0.004 | 0.028 | 0.001 | |
| C | Before | 16846 | 41.149 | 3952.034 | 1.387 |
| After | 16770 | 40.321 | 3910.396 | 1.371 | |
| Absolute difference | 76 | 0.8287 | 41.638 | 0.0157 | |
| Ratio | 0.0045 | 0.0203 | 0.0106 | 0.0114 | |
| Average ratio of 24 objects | 0.0285 | 0.0089 | 0.0233 | 0.0091 | |
For the second column, ‘type of information’, we showed the features before and after the straightening, as well as the absolute value of the difference, and the ratio defined as difference/(0.5*(before+after)). Also showed are the overall statistics of 24 image objects randomly selected from 3D images of three C.elegans individuals and measured in the same way.