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Positive nESI spectrum of myoglobin in 100% H2O with a) no acid vapor, b) formic acid vapor, c) trifluoroacetic acid vapor, d) hydrochloric acid vapor. The strength of the acids used increases c–f. (Peaks labeled with ○ correspond to holomyoglobin peaks, △ correspond to apomyoglobin peaks, and □ correspond to myoglobin with 2 heme groups.)
