Figure 2.—

Perithecial examination of various crosses demonstrates the requirement of qip in early sexual development. (A and B) qip⁺ × qip⁺ (F2-01 × P3-08). Normal perithecia and rosettes of eight-spored asci can be seen from the control cross. (C and D) qip^Δ × qip^Δ (F2-06 × P9-39). Undersized beaks (arrow in C) and the absence of asci (perithecial cross section in D) in a cross homozygous for qip^Δ. Bars, 500 μm. (E) Only paraphysal tissue is found in perithecia from a qip^Δ × qip^Δ (F2-06 × P9-39) cross, suggesting a severe defect in perithecial development in a qip-null background. DAPI stain was used. Bar, 10 μm.