. 2010 Sep;186(1):119–126. doi: 10.1534/genetics.110.118273

TABLE 2.

qipΔ, unlike Sad-1Δ, does not act as a dominant suppressor of MSUD

Unpaired gene	Parent 1 (mat A): ectopic insertion at his-3 (∷) and/or deletion (Δ)	Parent 2 (mata): mutation at sad-1 or qip	Ascospore count/phenotype	Predominant ascus phenotype
actin	∷act⁺	Wild type	208 × 10³	Lollipop asci
	∷act⁺	qip^Δ	320 × 10³	Lollipop asci
	∷act⁺	Sad-1^Δ	2408 × 10³	Normal
Ascospore maturation-1	∷asm-1⁺; Asm-1^Δ	Wild type	1.6% black	White ascospores
	∷asm-1⁺; Asm-1^Δ	qip^Δ	1.4% black	White ascospores
	∷asm-1⁺; Asm-1^Δ	Sad-1^Δ	86% black	Black ascospores
β-tubulin	∷Bml^R	Wild type	2.9 × 10³	Arrests before metaphase
	∷Bml^R	qip^Δ	2.4 × 10³	Arrests before metaphase
	∷Bml^R	Sad-1^Δ	4008 × 10³	Normal
Round spore	R^Δ	Wild type	0% football	Round ascospores
	R^Δ	qip^Δ	0% football	Round ascospores
	R^Δ	Sad-1^Δ	100% football	Football-shaped ascospores

Meiotic silencing of meiotically important genes, such as act⁺, asm-1⁺, Bml^R, and r⁺, leads to the reduced production of black (mature), American football (spindle)-shaped ascospores. Crosses carrying Sad-1^Δ, not qip^Δ, can improve the production of normal ascospores. Strains used in this experiment include F2-29, F2-35, F2-36, F3-23, P3-08, P3-25, and P9-39.