TABLE 1.

Description of our notation

Symbol or notation	Description
k	No. of haploid individuals used for separate sequencing
λ	Expected no. of times a locus is read for an individual using separate sequencing
n	Size of the pool in a pooling experiment
J	Random no. of individuals for which reads are actually available at a particular locus with individual sequencing (J ≤ k)
M	Random no. of reads for a particular locus in a pooling experiment [E(M) = kλ]
p	Relative frequency of the allele of interest in the population
F(P)(b, γ)	Poisson cumulative distribution function ()
F(B)(x, M, p)	Binomial cumulative distribution function
	Bias-corrected version of Tajima's π for a pooling experiment when the minor allele frequency is required to be at least b. For b = 1,
	Bias-corrected version of Watterson's θ for a pooling experiment when the minor allele frequency is required to be at least b ≥ 1