Figure 9.—

MAF2 artificial miRNA (MAF2 amiRNA) does not reduce time to flowering in the WS or Tu-1 accessions. (A) The accession Ler, which bears an active allele of MAF2, and WS and Tu-1, which bear MAF2 insertion alleles, were transformed with an empty vector control construct (solid bars) or the MAF2 amiRNA construct (shaded bars). Flowering time was recorded under long- and short-day conditions as rosette leaf number at flowering. (B) RT–PCR of MAF2 expression, using primers 44 and 45 in Ler, WS, and Tu-1 lines transformed with the empty vector or the MAF2 amiRNA construct.