Number of informative families based on expected heterozygosity, between pairs of parental lines plotted against the minor allele frequencies for 11 resequenced amplicons sampled from the worldwide collection of maize. The set of 27 maize inbred lines includes (1) A272, (2) D940Y, (3) EP1, (4) F2, (5) I205, (6) T232, (7) B103, (8) B97, (9) CI187-2, (10) Ki21, (11) Ky21, (12) M162W, (13) Mo17, (14) NC260, (15) Oh43, (16) Pa91, (17) W153R, (18) II101, (19) P39, (20) N28Ht, (21) A6, (22) CML254, (23) CML258, (24) CML333, (25) Ki3, (26) NC348, and (27) Tx601 (Liu et al. 2003). Sequences from the 27 lines are compared with B14A (A), Ia2132 (B), and IDS28 (C). The 27 lines also were randomly divided into three subgroups. (D) Group 1 includes 1, 4, 7, 10, 13, 16, 19, 22, and 25. Group 2 includes 2, 5, 8, 11, 14, 17, 20, 23, and 26. Group 3 includes 3, 6, 9, 12, 15, 18, 21, 24, and 27. Group 1 is compared with B14A, group 2 with Ia2132, and group 3 with IDS28 and the number of informative families were tallied across all three.