Figure 1.—

Partial depletion of SYP-1 by RNAi. (A) DAPI-stained chromosomes in oocytes at diakinesis, the last stage of meiotic prophase. Wild-type oocyte, six bivalents; syp-1 null mutant oocyte, 12 univalents; syp-1 RNAi oocyte, mixture of bivalents and univalents. (B) Immunolocalization of SYP-1 protein in nuclei from the midpachytene regions of germ lines from control and syp-1 RNAi worms. SYP-1 is localized along the full lengths of all chromosome pairs in the control pachytene nuclei. In the syp-1 RNAi nuclei, SYP-1 stretches are associated with only a subset of chromosomes and appear less uniform along their lengths than SYP-1 stretches in control nuclei. (C) Immunolocalization of DNA strand exchange protein RAD-51 in nuclei from the midpachytene (left) and late pachytene (right) regions of control and syp-1 RNAi germ lines; persistence of RAD-51 foci into the late pachytene region of the syp-1 RNAi germ line reflects reduced SYP-1 function. Bar, 5 μm.