TABLE 1.
Efficacy of syp-1 RNAi
| % oocytes with the indicated number of DAPI-stained bodies |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Strain/cross | 6 | 7 | 8 | 9 | 10 | 11 | 12 | n | Mean |
| a. Oocytes scored in worms exposed to feeding RNAi beginning at L1 stage | |||||||||
| N2 | 99 | 0 | 0 | 0 | 1 | 0 | 0 | 132 | 6.0 |
| AV335 | 3 | 2 | 8 | 18 | 29 | 28 | 12 | 246 | 10.0 |
| EU1067 | 54 | 0 | 8 | 11 | 13 | 11 | 2 | 61 | 7.7 |
| AZ212 | 0 | 1 | 0 | 5 | 35 | 44 | 15 | 55 | 10.6 |
| AZ244 | 67 | 6 | 6 | 10 | 7 | 3 | 0 | 67 | 6.9 |
| b. Oocytes scored in F1 progeny of indicated cross; feeding RNAi initiated when parents were at L4 stage | |||||||||
| AZ212 × Haw | 45.4 | 15.7 | 14.0 | 7.5 | 9.4 | 6.7 | 1.6 | 627 | 7.1 |
| c. Oocytes scored in the subset of F1 worms used for crossover analysis; feeding RNAi initiated when parents were at L4 stage | |||||||||
| AZ212 × Hawa | 3 | 18 | 47 | 23 | 8 | 0.7 | 0.7 | 144 | 8.2 |
a
The oocytes scored for c were from the F1 worms whose progeny were genotyped for the recombination analysis. All F2 worms genotyped were from plates on which abundant embryos laid by the F1 hermaphrodite (during the 12-hr egg-laying window) gave rise to <10 viable F2 progeny; a high proportion of inviable embryos was used as an indicator of effective syp-1 RNAi. Twenty percent of the viable F2 progeny of these F1's were males, indicative of X chromosome missegregation resulting from SYP-1 depletion.