Figure 3.—

Expression of Or47b–GAL4 truncation constructs. (A) Scheme of Or47b–GAL4 truncation constructs. Black lines represent Or47b sequences upstream of the predicted translation start site, and green arrows represent vector and GAL4 coding sequences. (B–D) Images are interior sections of antennae (top row) or maxillary palps (bottom row) heterozygous for the indicated construct and the UAS–GFP reporter, except Or47b−7.9kb, which is homozygous for both. (B) Constructs with 419 bp or more of Or47b upstream DNA show similar patterns of expression. (C) Variable expression patterns of truncated Or47b–GAL4 constructs. For each construct, confocal Z-projections from two independent insertion lines are shown. All lines of these three constructs exhibited some degree of maxillary palp GFP expression. (D) No expression was observed in antennae or maxillary palps of flies carrying Or47b−119. (E–G) Antennal lobes showing neuropil (nc82 antibody) in magenta and GFP expression (anti-GFP antibody) in green. (E) Antennal lobe from a 0- to 1-day-old fly homozygous for Or47b−7.9kb–GAL4 and UAS–GFP. (F and G) Antennal lobes from 7- to 8-day-old flies heterozygous for the indicated Or47b−219 insertion and the UAS–GFP reporter. Similar patterns of glomerular targeting were seen in 0- to 1-day-old flies (not shown). Abbreviations: P, proximal; Do, dorsal; Di, distal; M, medial; L, lateral; V, ventral.