Figure 8.—

Mutation of two GCAATTA elements upstream of Or88a. (A) Constructs. Each Or88a promoter fragment extends to, but does not include, the ATG of Or88a. Hatched lines represent downstream Or88a promoter sequence; green arrows represent vector and GAL4 coding sequence. Mutated base pairs are in lowercase type and shaded, while the positions of three iterations of GCAATTA are underlined. (B) Confocal Z-projections of 0- to 1-day male antennae heterozygous for both the indicated Or88a–GAL4 construct and the UAS–GFP reporter. (C–F) Numbers of labeled sensilla in flies heterozygous for one of the Or88a–GAL4 constructs in A and the UAS–GFP reporter. 5 ≤ n ≤ 13 antennae for all lines except that for −486M4, line 1 and −465M1, line 1, n = 3. The scales in C and D are different from those in E and F. 0 indicates that no GFP-labeled sensilla were detected. Abbreviations: nd, not determined; d, days post-eclosion; P, proximal; M, medial; L, lateral; Di, distal.