TABLE 2.
Or67d reporter construct expression
| Construct name | Upstream DNA (bp) | Lines examined | Antennal ORN expressiona | Maxillary palp expressionb |
|---|---|---|---|---|
| Or67d−6.1kb | 6138 | 10 | +++ | +c |
| Or67d−736 | 736 | 3 | +++ | — |
| Or67d−636 | 636 | 2 | +++ | — |
| Or67d−536 | 536 | 3 | +++ | +++ |
| Or67d−436 | 436 | 3 | +++ | +++ |
| Or67d−336 | 336 | 4 | +++ | +++ |
| Or67d−236 | 236 | 4 | +++ | +++ |
| Or67d−136 | 136 | 2 | — | — |
a
Qualitative assessment of expression level. We observe GFP-labeled ORNs in both trichoid and nontrichoid sensilla; +++ indicates the highest number of GFP-labeled olfactory sensilla and — indicates no GFP-labeled sensilla. All constructs except 67d−136 show expression in nonneuronal antennal cells.
b
+++ indicates consistent GFP expression and — indicates GFP expression in no more than one maxillary palp among all lines examined.
c
We analyzed maxillary palp expression in one line, which was homozygous for both Or67d−6.1kb and the UAS-GFP reporter, and observed modest GFP expression.