TABLE 3.
Or65a reporter construct expression
| Construct name | Upstream DNA (bp)a | Lines examined | Antennal ORN expressionb | Maxillary palp expression |
|---|---|---|---|---|
| Or65a−4.7kb | 4665 | 3 | ++++ | — |
| Or65a−637 | 636 | 2 | ++ | — |
| Or65a−537 | 536 | 2 | ++ | — |
| Or65a−437 | 436 | 5 | ++ | — |
| Or65a−387 | 386 | 7 | +++ | — |
| Or65a−238 | 237 | 5 | ++ | — |
| Or65a−137 | 136 | 2 | — | — |
a
The Or65a anchor primer (Ejima et al. 2007) extends to −2 relative to the Or65a translation start site; thus, the amount of upstream DNA included in each truncation is 1 bp shorter than suggested by the construct name.
b
Qualitative assessment of expression level. We observe GFP-labeled ORNs in both trichoid and nontrichoid sensilla; ++++ indicates the highest number of GFP-labeled olfactory sensilla and — indicates no GFP-labeled sensilla.