TABLE 4.
Construct name | Upstream DNA (bp) | Lines examined | Trichoid ORN expressiona | Maxillary palp expression |
---|---|---|---|---|
Or88a−1.6kb | 1621 | 6 | ++++ | — |
Or88a−486 | 486 | 6 | ++ | — |
Or88a−486M1 | 486 | 3 | +++ | — |
Or88a−486M2 | 486 | 4 | +++ | — |
Or88a−486M2+3 | 486 | 6 | +++ | — |
Or88a−486M4 | 486 | 5 | — | — |
Or88a−486D | 479 | 5 | +++ | — |
Or88a−473 | 472b | 3 | ++ | — |
Or88a−465 | 465 | 5 | ++ | — |
Or88a−465M1 | 465 | 7 | — | — |
Or88a−359 | 360 | 1 | — | — |
Or88a−159 | 160 | 1 | — | — |
Qualitative assessment of expression level; ++++ indicates highest number of GFP-labeled trichoid sensilla, — indicates no GFP-labeled sensilla.
This construct extends to position −473 of the Or88a upstream region but carries a −458A deletion, making the total construct length 472 bp.