FIG. 1.
LCM of rat seminiferous tubules. Rat testis were cut on a cryostat at 8 μm thickness and processed for LCM. (A) Micrograph of intact testis section prior to LCM. (B1) Group 1 (stages I–VI): arrows indicate elongated spermatid heads embedded within the seminiferous epitheliums. (B2) Group 2 (stages VII–VIII): arrows indicate elongated spermatid heads adjacent to the tubular lumen. (C) Remaining tissue section after LCM. (D) Isolated tissue on the LCM cap. The scale bar is 100 um in A, C, and D, and the scale bar is 50 um in B1 and B2.