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Mitochondrial function in primary hepatocytes after treatment with 5mM APAP for 6 or 15 h at various oxygen concentrations. Mitochondrial function (A) and membrane potential (B) were determined by reduction of XTT and JC-1 fluorescence, respectively. Data are given as percentage compared with untreated control at each oxygen concentration and time point. Values represent mean ± SE of n = 3 independent experiments. *p < 0.05 (compared with APAP-treated samples at 21% oxygen).
