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Mitochondrial reactive oxygen production after treatment with APAP for 6 or 15 h at 21 or 10% oxygen. Primary mouse hepatocytes were isolated and treated with APAP; cells were loaded with Mitosox after 6 or 15 h, lysed, and fluorescence measured on a spectrofluorometer. Data are expressed as mean ± SE of three independent experiments. *p < 0.05 (compared with respective control) and #p < 0.05 (compared with 21% oxygen).
