FIG. 1.

Histological analysis of the effects of treatment with cadmium chloride, MAA, and DNB on rat testes. Rats were dosed with either a low or high dose of toxicant and testes collected 24 h later and evaluated for immunoexpression of the germ cell–specific protein DAZL (brown); counterstaining used hematoxylin (blue). Scale bar represents 100 μm. (A, D, and G). Testes from vehicle-treated rats show normal spermatogenesis and the presence of DAZL in pachytene spermatocytes and residual bodies. Testis sections from rats treated 24 h earlier with a low dose of toxicant, (B) 1 mg/kg CdCl₂, (E) 200 mg/kg MAA, (H) 25 mg/kg DNB or a high dose of toxicant, (C) 3 mg/kg CdCl₂, (F) 650 mg/kg MAA, and (I) 50 mg/kg DNB show dose-related toxicant-induced damage to spermatogenesis.