FIG. 4.

Localization of BTB proteins occludin (red) and ZO-1 (green) (arrows, panels A and B and insert, panel A) and N-cadherin (red) and β-catenin (green) (panels C and D), in testes from vehicle-treated control rats (A and C) and rats treated 24 h earlier with 3 mg/kg cadmium chloride (B and D). Note the depletion of all BTB proteins from the site of the BTB following treatment with 3 mg/kg cadmium chloride. Scale bar represents 50 μm. Blue = nuclear counterstain (Dapi). Note also that the red “staining” present in the interstitium represents nonspecific binding of the tyramide Cy3 as it was also present in slides for which the occludin antibody was omitted (not shown).