FIG. 5.

Localization of biotin tracer (green) in testes from a vehicle-treated control rat (A) and rats treated with either 3 mg/kg CdCl₂ (B), 650 mg/kg MAA (C), or 50 mg/kg DNB (D). Occludin protein is shown in red to indicate the site of the BTB. The biotin tracer is localized to the interstitium and up to the site of the BTB in control testes, and testes from animals treated with 650 mg/kg MAA or 50 mg/kg DNB (A, C, and D) but has penetrated the STs following treatment with 3 mg/kg CdCl₂ (B). Scale bar represents 50 μm. Blue= nuclear counterstain (Dapi).