Fig. 1.

Sequence of MBP2 and location of mutations. Mutations are indicated in bold under the amino acid sequence. Δ2682 indicates a deletion of a thymine at position 2682 in the DNA sequence, which leads to a frameshift of the subsequent residues in MBP2. Secondary structural elements are indicated above the sequence, arrows for β-sheets and helices for α-helices, according to the PDB file 1ANF (Spurlino et al. 1991). The β-sheets are labeled with a letter to indicate the structural element and a number to indicate the strand in that element; e.g., β-sheet “A” is made up of two strands, A1 and A2. Helices are indicated by a number. The numbering of the mutations we obtained ignores the N-terminal methionine present in MBP2, to simplify comparison to the structure and the previous literature. The sequences corresponding to the hinges between the two domains are indicated as bold letters embedded in the sequence