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Dissociation constants of a subset of the mutant proteins
| Kd maltose | Kd maltotriose | |
|---|---|---|
| MBP2 | 1.49 ± .01 | 0.38 ± .02 |
| F67L | 7.00 ± .7 | 1.40 ± .2 |
| A312V | 0.23 ± .02 | 0.11 ± .01 |
| I317V | 0.60 ± .08 | 0.20 ± .03 |
| Q325R | 0.52 ± .05 | 0.31 ± .02 |
| V343M | 0.92 ± .06 | 0.54 ± .05 |
| A312V I317V | 0.16 ± .02 | 0.03 ± .01 |
| A312V I317A | 0.86 ± .06 | 0.19 ± .03 |
Values are calculated as described in “Materials and methods”; error is calculated from the curve-fitting software, and is not an experimental error
