Table 1.
Primers used for PCR
| Gene | Primer name | Sequence | Tm (C) | nt |
|---|---|---|---|---|
| mGAPDH | mGAPDH-for | TTGTCAGCAATGCATCCTGCACCACC | 61 | 119 |
| NM_008084.2 | mGAPDH-rev | CTGAGTGGCAGTGATGGCATGGAC | 61 | |
| mAqp1 | mAqp1-top | CACTGTGCCCTTAACCACATTGTGAACC | 61 | 128 |
| NM_007472.1 | mAqp1-bot | GGGAACGGAGACGAGTGTGCAGC | 62 | |
| mSlpi | mSlpi-top | GGTTCCATGGCTCCCGGCTCC | 62 | 127 |
| NM_011414.2 | mSlpi-bot | CTCTCCAACAGCATTTCCCTAAAGATCGG | 62 | |
| mGAPDH | mGAPDH-Lt | TTGTCAGCAATGCATCCTGCACCAC | 59 | 412 |
| NM_008084.2 | mGAPDH-Lb | GAGACAACCTGGTCCTCAGTGTAG | 62 | |
| mAqp1 | mAqp1-Lt | GCTGCACACTCGTCTCCGTTCCCTAGCAG | 67 | 316 |
| NM_007472.1 | mAqp1-Lb | CATACACAGAGCAGACAGATACAAGGTCCTGGTTC | 63 | |
| mSlpi | mSlpi-Lt | CCCTCCCAATGTCTGCCAGAGGGAC | 64 | 309 |
| NM_011414.2 | mSlpi-Lb | TGCTCTCCAACAGCATTTCCCTAAAGATCGG | 63 |
for, Forward; rev, reverse; bot, bottom; Tm, temperature; nt, nucleotide.