Skip to main content
. 2010 Jun 23;24(8):1512–1528. doi: 10.1210/me.2009-0320

Figure 7.

Figure 7

E-box 3 (-397CACATG-392) is required to mediate inhibition of IL-8 promoter activity in cervical stromal cells. A, IL-8 promoter sequences with E-box 3 sequences underlined. Sequence of the mutated IL-8 promoter within E-box 3 is noted. Transient transfection assays in human cervical stromal cells. A 770-bp IL-8 promoter luciferase reporter construct (IL-8) with wild-type or mutated E-box 3 (IL-8mut) was transfected alone or together with indicated combinations of expression vectors encoding MiTF-CX. Total DNA in each transfection was equivalent. Relative luciferase activity was normalized to that of β-galactosidase and presented as mean ± sem of four samples in each group. *, P < 0.05 compared with MiTF-CX-stimulated wild-type (WT) IL-8 reporter. B, ChIP assays in cervical stromal cells infected with control, MiTF-CX, or MiTF-CXmi adenovirus. MiTF and mutated MiTF were bound to chromatin complexes associated with E-box 3 in the IL-8 promoter. MiTF did not bind to E-box 10 (negative control).