Table 2.
Primer sequences to amplify MiTF isoforms using conventional PCR
| MiTF isoform | GenBank accession no. | Forward primer | Reverse primer (NM000248) | Size |
|---|---|---|---|---|
| A | AB006909 | 142cggatttcgaagtcggggaggagt165 | 181ggtggggttttcgaggtgggtctg157 | 356 |
| H | AB032358 | 4113tggaggcgcttagagttca4132 | 208ccgttgggcttgctgtat190 | 389 |
| MCa | NT022459 | 3594513agtagcaggggttagtaggtggat3594536 | 180gtggggttttcgaggtgggtctg157 | 404 |
| C | AB006988 | 58ggttttcccacgagctattttct80 | 180gtggggttttcgaggtgggtctg157 | 351 |
| M | NM000248 | 58agaaagtagagggagggatagtct82 | 208ccgttgggcttgctgtat190 | 150 |
| common | NM000248 | 320ggagcagcgcacccaacag339 | 533tcaagcccaagatttcctcattat509 | 213 |
| CX | atttctgcacctcccgttctattg | 180gtggggttttcgaggtgggtctg157 | 341 |
GenBank accession numbers for isoform-specific forward primers are listed. Reverse primers were derived from sequence no. NM_000248.
Human homolog of mouse MC isoform, chromosome 3p genomic sequence.