Figure 2.

Isolation and sequencing of full-length mGRβ mRNA. A, Genomic organization (not to scale) of the mGR gene, showing location of forward primer (exon 1) and reverse primers (1 and 2) used for RT-PCR analysis. B, Primer 3 in conjunction with the forward primer in exon 1 was used to generate a second full-length mGRβ product that was cloned and sequenced to yield the organization of mGRβ mRNA is seen in C. Primer 3 in conjunction with the forward primer in exon 2 was used to generate the cDNA cloned into pcDNA3.1+ (see Fig. 5). Con, Amplification without template.