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. 2010 Jul 7;24(9):1765–1781. doi: 10.1210/me.2009-0487

Figure 2.

Figure 2

LHR signaling decreases cofilin phosphorylation in vitro and in vivo. A, Preovulatory granulosa cells from PMSG-primed rats were isolated and plated overnight in complete medium. Cells were then left untreated (- - -) or treated with 1 IU/ml hCG for indicated times. Western blots are representative of five separate experiments. Graphs represent densitometric analysis of phospho (p)-cofilin Western values divided by total cofilin values to account for loading differences. Values are expressed as fold change from that of the untreated lysates, which has been set to a value of one. Values are the mean ± se of five separate experiments. *, P < 0.01; #, P > 0.01. B, PMSG-primed rats were injected with 50 IU hCG or not treated (0 h). Ovaries were harvested at specific times after injection, and whole ovarian extracts were prepared according to Materials and Methods. Clarified lysates were used for SDS-PAGE. Results for 10- and 30-min time points are from one experiment and results for 1- and 5-h time points are representative of two separate experiments. Graphs represent densitometric analysis of phospho-cofilin divided by total cofilin Western values to account for loading differences. Values are expressed as fold change from that of 0 h, which has been set to a value of one. Values are the mean ± sd of two separate experiments.