Figure 4.

E2 enhances the inhibitory effects of diazoxide on the firing of GnRH neurons. A–C, Representative recordings of the induced firing under control conditions (A) and in the presence of diazoxide before (B) and after treatment with 100 nm E2 for 15 min (C). D, Current-clamp protocol used to induce firing. Cells were initially held at −60 mV, and a series of current pulses of 5–45 pA with a duration of 2 sec were applied to induce firing. E, Summary of the enhancing effects of E2 on the ability of diazoxide (50 μm) to inhibit the induced firing from six GnRH neurons. **, P < 0.01 compared with control group; #, P < 0.05 and ##, P < 0.01 compared with diazoxide group.