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. 2010 Jun 23;151(9):4494–4503. doi: 10.1210/en.2010-0223

Figure 7.

Figure 7

Confocal dual immunofluorescence projections (magnification, ×10; 1-μm optical sections) of a pair of coronal hemi-MBH sections taken at the level of the arcuate nucleus (ARC)-median eminence (ME) region from each of four castrated adult male monkeys stained for NKB (red fluorescence, Cy3) and kisspeptin (green fluorescence, Alexa 488). The left hand section of each pair (A–D) is anterior with respect to the more caudal section shown on the right (A′–D′). Note that colocalization of the two peptides (yellow) in approximately 40–60% of kisspeptin positive neurons of the ARC. 3V, Third ventricle. Scale bar, 100 μm.