Figure 8.

A and B, Confocal immunofluorescence projections showing the distribution of NKB fibers (red fluorescence, Cy3) in relation to GnRH axons (green fluorescence, Alexa 488) in coronal hemisections of the median eminence (ME) of two castrated adult male monkeys (magnification, ×10; 1-μm optical sections). A′ and B′, Confocal projections (magnification, ×100; 1-μm optical sections) of NKB-GnRH interactions in regions of the internal zone of the median eminence shown in the area outlined by the rectangles in A and B, respectively. Contacts between NKB and GnRH fibers (white arrows) were confirmed by examination of individual optical sections. 3V, Third ventricle; ARC, lateral arcuate nucleus. Scale bars, 100 μm (A and B) and 10 μm (A′ and B′).