Table 1.
Femoral histomorphometry of 1-, 4-, and 6-month-old male and female Ctgf+/LacZ heterozygous mice and wild-type controls
| 1 month
|
4 months
|
6 months
|
||||
|---|---|---|---|---|---|---|
| Wild type | Ctgf+/LacZ | Wild type | Ctgf+/LacZ | Wild type | Ctgf+/LacZ | |
| Males | ||||||
| Bone volume/tissue volume (%) | 7.7 ± 0.7 | 5.2 ± 0.6a | 9.2 ± 0.7 | 7.7 ± 0.8 | 7.1 ± 1.1 | 6.7 ± 1.1 |
| Trabecular separation (μm) | 292 ± 25 | 482 ± 70a | 252 ± 19 | 266 ± 13 | 409 ± 51 | 372 ± 33 |
| Trabecular number (mm−1) | 3.4 ± 0.2 | 2.5 ± 0.2a | 3.7 ± 0.2 | 3.5 ± 0.2 | 2.5 ± 0.3 | 2.6 ± 0.2 |
| Trabecular thickness (μm) | 22.2 ± 1.0 | 20.8 ± 0.6 | 24.7 ± 0.6 | 23.2 ± 0.6 | 27.4 ± 1.7 | 25.4 ± 2.6 |
| Osteoblast surface/bone surface (%) | 24.5 ± 1.1 | 25.9 ± 1.5 | 16.4 ± 1.5 | 18.4 ± 1.4 | 15.5 ± 1.2 | 14.2 ± 0.6 |
| Number of osteoblasts/bone perimeter (mm−1) | 27 ± 1 | 30 ± 2 | 13.3 ± 1.4 | 15.0 ± 1.1 | 11.9 ± 1 | 11.1 ± 1 |
| Number of osteoblasts/tissue area (mm−2) | 142 ± 9 | 112 ± 11a | 77 ± 7 | 82 ± 5 | 47 ± 8 | 45 ± 4 |
| Osteoclast surface/bone surface (%) | 16.1 ± 0.6 | 17.0 ± 0.6 | 4.4 ± 0.4 | 4.8 ± 0.5 | 5.2 ± 0.4 | 5.6 ± 0.5 |
| Number of osteoclasts/bone perimeter (mm−1) | 7.3 ± 0.3 | 8.2 ± 0.2 | 2.1 ± 0.2 | 2.3 ± 0.2 | 2.2 ± 0.1 | 2.5 ± 0.2 |
| Number of osteoclasts/tissue area (mm−2) | 43 ± 3 | 32 ± 3a | 12 ± 1 | 13 ± 2 | 9 ± 1 | 10 ± 1 |
| Eroded surface/bone surface (%) | 28 ± 1 | 29 ± 1 | 10 ± 1 | 11 ± 1 | 10 ± 1 | 11 ± 1 |
| Mineral apposition rate (μm/d) | 2.20 ± 0.11 | 2.38 ± 0.09 | 0.77 ± 0.04 | 0.85 ± 0.04 | 0.64 ± 0.03 | 0.70 ± 0.04 |
| Mineralizing surface/bone surface (%) | 1.86 ± 0.35 | 2.56 ± 0.28 | 3.69 ± 0.55 | 3.73 ± 0.49 | 3.43 ± 0.63 | 3.15 ± 0.91 |
| Bone formation rate (μm3/μm2/d) | 0.043 ± 0.009 | 0.061 ± 0.007 | 0.029 ± 0.005 | 0.032 ± 0.005 | 0.023 ± 0.004 | 0.023 ± 0.007 |
| Females | ||||||
| Bone volume/tissue volume (%) | 7.1 ± 0.6 | 4.0 ± 0.8a | 3.1 ± 3.3 | 3.3 ± 0.3 | 3.0 ± 0.4 | 3.2 ± 0.4 |
| Trabecular separation (μm) | 273 ± 31 | 468 ± 69a | 695 ± 62 | 631 ± 54 | 752 ± 97 | 704 ± 81 |
| Trabecular number (mm−1) | 3.6 ± 0.3 | 2.3 ± 0.4a | 1.5 ± 0.2 | 1.6 ± 0.1 | 1.4 ± 0.2 | 1.4 ± 0.1 |
| Trabecular thickness (μm) | 19.9 ± 0.8 | 17.2 ± 1.2 | 19.8 ± 0.4 | 20.1 ± 0.9 | 21.5 ± 0.7 | 21.7 ± 1.1 |
| Osteoblast surface/bone surface (%) | 27.7 ± 1.7 | 27.3 ± 2.1 | 23.3 ± 2.1 | 17.8 ± 2.2 | 26.6 ± 3.2 | 21.0 ± 1.6 |
| Number of osteoblasts/bone perimeter (mm−1) | 31 ± 1 | 32 ± 3 | 20 ± 2 | 14 ± 2 | 19.7 ± 2.5 | 16.0 ± 1.1 |
| Number of osteoblasts/tissue area (mm−2) | 171 ± 14 | 110 ± 10a | 47 ± 5 | 34 ± 3a | 41 ± 5 | 36 ± 5 |
| Osteoclast surface/bone surface (%) | 16.0 ± 0.9 | 15.6 ± 0.7 | 8.4 ± 0.6 | 9.7 ± 0.5 | 6.8 ± 0.7 | 7.4 ± 1.4 |
| Number of osteoclasts/bone perimeter (mm−1) | 8.0 ± 0.4 | 7.9 ± 0.4 | 4.3 ± 0.3 | 4.9 ± 0.3 | 2.8 ± 0.3 | 3.2 ± 0.7 |
| Number of osteoclasts/tissue area (mm−2) | 44 ± 3 | 28 ± 5a | 10 ± 1 | 12 ± 1 | 6 ± 1 | 7 ± 1 |
| Eroded surface/bone surface (%) | 29 ± 2 | 29 ± 1 | 18 ± 1 | 22 ± 0 | 13 ± 1 | 15 ± 3 |
| Mineral apposition rate (μm/d) | 2.12 ± 0.14 | 2.13 ± 0.36 | 1.01 ± 0.11 | 1.14 ± 0.07 | 1.11 ± 0.04 | 1.14 ± 0.07 |
| Mineralizing surface/bone surface (%) | 3.9 ± 0.6 | 3.8 ± 0.5 | 1.84 ± 0.40 | 1.57 ± 0.29 | 6.68 ± 1.61 | 10.55 ± 1.03 |
| Bone formation rate (μm3/μm2/d) | 0.084 ± 0.014 | 0.080 ± 0.009 | 0.019 ± 0.004 | 0.018 ± 0.003 | 0.076 ± 0.020 | 0.118 ± 0.009 |
Bone histomorphometry was performed on femurs from 1-, 4-, and 6-month-old male and female Ctgf+/LacZ heterozygous mice and wild-type littermate controls. Values are means ± sem (n = 5–13).
a
Significantly different from controls, P < 0.05 by unpaired t test.