Table 3.
Femoral histomorphometry of 1-, 4-, and 6-month-old male and female Oc-Cre/+;CtgfINV/LacZ conditional null mice and controls
| 1 Month
|
4 Months
|
6 Months
|
||||
|---|---|---|---|---|---|---|
| CtgfINV/+ | CtgfINV/LacZ | CtgfINV/+ | CtgfINV/LacZ | CtgfINV/+ | CtgfINV/LacZ | |
| Males | ||||||
| Bone volume/tissue volume (%) | 10.0 ± 1.4 | 9.2 ± 1.0 | 7.6 ± 1.2 | 6.4 ± 0.7 | 8.3 ± 0.8 | 5.4 ± 0.6a |
| Trabecular separation (μm) | 205 ± 27 | 234 ± 27 | 328 ± 51 | 347 ± 26 | 305 ± 21 | 444 ± 43a |
| Trabecular number (mm−1) | 4.6 ± 0.6 | 4.3 ± 0.4 | 3.0 ± 0.4 | 2.8 ± 0.2 | 3.1 ± 0.2 | 2.3 ± 0.2a |
| Trabecular thickness (μm) | 21.7 ± 1.4 | 21.6 ± 0.6 | 25.1 ± 1.7 | 22.8 ± 1.2 | 26.6 ± 1.6 | 23.4 ± 1.2 |
| Osteoblast surface/bone surface (%) | 22.4 ± 1.8 | 20.2 ± 1.3 | 12.6 ± 0.9 | 13.4 ± 1.2 | 15.0 ± 1.8 | 18.1 ± 1.0 |
| Number of osteoblasts/bone perimeter (mm−1) | 24.4 ± 2.5 | 22.0 ± 1.5 | 12.2 ± 0.8 | 13.5 ± 1.0 | 11.9 ± 1.4 | 15.0 ± 1.1 |
| Number of osteoblasts/tissue area (mm−2) | 183 ± 40 | 145 ± 15 | 58 ± 10 | 58 ± 5 | 57 ± 7 | 52 ± 4 |
| Osteoclast surface/bone surface (%) | 13.4 ± 1.6 | 14.4 ± 0.8 | 8.4 ± 0.7 | 9.9 ± 0.7 | 4.6 ± 0.5 | 5.2 ± 0.5 |
| Number of osteoclasts/bone perimeter (mm−1) | 5.9 ± 0.7 | 6.4 ± 0.4 | 4.8 ± 0.4 | 5.5 ± 0.3 | 2.1 ± 0.2 | 2.5 ± 0.2 |
| Number of osteoclasts/tissue area (mm−2) | 41 ± 3 | 42 ± 4 | 22 ± 2 | 24 ± 3 | 10 ± 1 | 9 ± 1 |
| Eroded surface/bone surface (%) | 21.8 ± 2.0 | 25.0 ± 1.6 | 23.2 ± 2.3 | 25.9 ± 1.1 | 10.5 ± 1.1 | 11.6 ± 1.1 |
| Mineral apposition rate (μm/d) | 1.93 ± 0.20 | 1.86 ± 0.09 | 0.62 ± 0.06 | 0.50 ± 0.05 | 0.60 ± 0.03 | 0.60 ± 0.03 |
| Mineralizing surface/bone surface (%) | 2.62 ± 0.60 | 3.68 ± 0.42 | 5.35 ± 2.14 | 3.47 ± 1.35 | 3.35 ± 0.71 | 2.08 ± 0.55 |
| Bone formation rate (μm3/μm2/d) | 0.053 ± 0.017 | 0.067 ± 0.006 | 0.03 ± 0.02 | 0.02 ± 0.01 | 0.020 ± 0.005 | 0.013 ± 0.004 |
| Females | ||||||
| Bone volume/tissue volume (%) | 3.8 ± 0.3 | 5.9 ± 0.7 | 3.4 ± 0.5 | 2.5 ± 0.7 | 1.8 ± 0.4 | 2.6 ± 0.5 |
| Trabecular separation (μm) | 554 ± 32 | 366 ± 52a | 662 ± 73 | 913 ± 167 | 1188 ± 204 | 982 ± 237 |
| Trabecular number (mm−1) | 1.8 ± 0.1 | 2.8 ± 0.4 | 1.5 ± 0.2 | 1.2 ± 0.2 | 0.9 ± 0.1 | 1.2 ± 0.2 |
| Trabecular thickness (μm) | 21.6 ± 1.1 | 21.5 ± 0.7 | 22.2 ± 1.8 | 19.8 ± 1.5 | 18.6 ± 1.5 | 20.9 ± 2.0 |
| Osteoblast surface/bone surface (%) | 24.3 ± 3.5 | 21.8 ± 1.1 | 16.1 ± 1.5 | 16.7 ± 4.7 | 19.1 ± 2.1 | 16.7 ± 1.7 |
| Number of osteoblasts/bone perimeter (mm−1) | 25.0 ± 3.8 | 23.1 ± 1.2 | 16.2 ± 1.4 | 15.8 ± 3.8 | 22.0 ± 2.3 | 18.9 ± 1.8 |
| Number of osteoblasts/tissue area (mm−2) | 68 ± 10 | 102 ± 18 | 40 ± 7 | 33 ± 12 | 33 ± 7 | 36 ± 6 |
| Osteoclast surface/bone surface (%) | 15.4 ± 2.1 | 15.8 ± 0.7 | 9.4 ± 0.2 | 9.8 ± 0.8 | 13.3 ± 1.7 | 13.1 ± 0.8 |
| Number of osteoclasts/bone perimeter (mm−1) | 7.1 ± 1.0 | 7.0 ± 0.4 | 6.0 ± 0.2 | 6.8 ± 0.4 | 6.8 ± 0.8 | 6.3 ± 0.4 |
| Number of osteoclasts/tissue area (mm−2) | 19 ± 3 | 31 ± 5 | 14 ± 2 | 13 ± 3 | 10 ± 2 | 12 ± 2 |
| Eroded surface/bone surface (%) | 27.9 ± 3.4 | 25.6 ± 0.9 | 24.6 ± 0.5 | 28.6 ± 2.9 | 21.9 ± 2.5 | 23.3 ± 1.4 |
| Mineral apposition rate (μm/d) | 1.84 ± 0.01 | 2.14 ± 0.17 | 0.87 ± 0.05 | 0.99 ± 0.20 | 0.66 ± 0.08 | 0.81 ± 0.06 |
| Mineralizing surface/bone surface (%) | 5.09 ± 1.00 | 2.99 ± 0.19a | 6.02 ± 0.91 | 4.43 ± 1.03 | 12.45 ± 2.96 | 11.25 ± 1.77 |
| Bone formation rate (μm3/μm2/d) | 0.094 ± 0.019 | 0.064 ± 0.007 | 0.053 ± 0.01 | 0.035 ± 0.01 | 0.093 ± 0.031 | 0.10 ± 0.02 |
Bone histomorphometry was performed on femurs from 1-, 4-, and 6-month-old male and female Oc-Cre/+;CtgfINV/LacZ conditional null mice and Oc-Cre/+;CtgfINV/+ littermate controls. Values are means ± sem (n = 3–10).
a
Significantly different from controls, P < 0.05 by unpaired t test.