Figure 1.

Fasting induces HSL activation and lipolysis in adipose tissue. Ten-week-old C57BL/6 male mice were fasted at indicated times with free access to water. Refed mice were fasted for 24 h and refed for 4 h, after which blood and epididymal fat samples were collected. A, Serum FFA were measured. B, Protein was prepared from epididymal fat. Total and phosphorylated HSL protein levels were measured by Western blot analysis using specific antibodies. n = 6 per group. *, P < 0.05 vs. the fed group. Error bars indicate ±sem.