Figure 4.

Elevated B56α gene expression in epididymal fat of DIO mice. Protein and mRNA were extracted from the epididymal fat of control (Con) and DIO mice. A, PP2A catalytic subunits were captured by precoated specific antibody, and release of phosphate from a synthetic phosphopeptide substrate was measured. B, Protein levels of B56α and other indicated PP2A subunits were measured by Western blot analysis. Quantified data were from 10 mice. Error bars indicate ±sem. C, mRNA levels of B56 subunits were measured using real-time PCR. n = 10. *, P < 0.001 vs. adipose explants from control mice. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.