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. 2010 Jun 9;151(8):3624–3632. doi: 10.1210/en.2010-0245

Figure 5.

Figure 5

B56α/PP2A inhibits HSL activation and lipolysis in adipocytes. A, OA increases HSL Ser660 phosphorylation in 3T3-L1 adipocytes. Mature 3T3-L1 adipocytes were treated with OA (1 nm), and protein samples were collected at indicated times. HSL Ser660 phosphorylation and total HSL protein levels were measured by Western blot analysis. n = 6. *, P < 0.05; **, P < 0.01 vs. cells without OA treatment. Data are expressed as means ± sem. B–D, 3T3-L1CARΔ1 cells were differentiated into adipocytes and transduced with adenovirus encoding B56α or siRNA against B56α. Twenty-four hours after transduction, the cells were treated with 100 mm Bt2-cAMP. Total HSL and Ser660 phosphorylation were measured by Western blot analysis (B and C). FFA and free glycerol in the medium were measured (D). cAMP-stimulated increments of FFA or glycerol were analyzed. n = 6. Data are expressed as means ± sem. GFP, Green fluorescent protein.