. 2010 Jun 2;151(8):3874–3887. doi: 10.1210/en.2009-1356

Table 2.

Pathway analysis of nitrosoproteins in human umbilical vein endothelial cells: potential functionality

Biological functions	-log (P value)^a	S-NO-proteins identified
Cell viability/apoptosis	7.854–5.246	ACTB^b, APRT^b, ANXA1^b, ANXA5, ATP5H^b, EEF2^b, EIF5A^b, ENO1^b, FUBP1, GAPDH^b, GSTP1^b, HNRNPC, HSP90AA1, HSPA5, HSPB1, LGALS3^b, NME1^b, NP^b, P4HB, PARK7^b, PCNA, PPIA^b, PRDX2^b, PRDX6^b, SND1, STMN1^b, THBS1, TPT1^b
Cellular assembly and organization	4.216–1.325	ACTB^b, ANXA1^b, ANXA2^b, ANXA5, CFL1^b, GAPDH^b, LGALS3^b, VIM^b, SEC13^b, NME1^b, STMN1^b, PRDX6^b, HSP90B1, NP^b, PRDX2^b, HSP90AA1, HSPB1, THBS1
Cellular function and maintenance	4.152–1.457	NME1^b, THBS1, ANXA1^b, VIM^b, HSP90AA1, HSP90B1, HSPA5, HSPB1
Molecular transport	3.921–1.304	ANXA1^b, HSP90AA1, PRDX6^b, STMN1^b, THBS1, PARK7^b, APRT^b, LGALS3^b, TPT1^b, NME1^b, NP^b, GSTO1^b, GAPDH^b, ANXA5, PRDX2^b
Protein synthesis	3.347–1.544	HSP90B1, HSPA5, PARK7^b, PRDX6^b, HSP90AA1, P4HB, ANXA2^b, PARK7^b, PPIA^b, NME1^b, THBS1, EIF5A^b, HSPB1, EEF2^b, KRT7^b, PSMB3^b, UBC^b
Small molecule biochemistry	3.347–1.325	ANXA1^b, TKT^b, PRDX6^b, ANXA2^b, EIF6, THBS1, STMN1^b, GSTP1^b, APRT^b, PCNA, P4HB, NME1^b, PARK7^b, PPIA^b, PRDX2^b, ANAX5, NP^b, TPT1^b, GSTO1^b, GAPDH^b
Cell cycle	2.492–1.386	THBS1, VIM^b, FUBP1, PCNA, STMN1^b, PPIA^b, NME1^b, GSTP1^b, HSPB1, PRDX6^b, KRT7^b
Metabolism	2.492–1.325	TKT^b, PRDX6^b, THBS1, GSTO1^b, ANXA5, ANXA2^b, ENO1^b, PKM2, GAPDH^b, ANXA1^b, PRDX6^b, GSTP1^b, THBS1, PRDX2^b, STMN1^b, NME1^b, HSPA5, GSTO1^b, P4HB, APRT^b, PCNA, NME1^b, NP^b, TPT1^b
Cardiovascular system development and function	2.294–1.420	THBS1, LGALS3^b, PRDX6^b, TPT1^b, TKT^b, VIM^b, ANXA1^b, ANXA5
Gene expression	2.191–1.355	SND1, PCNA, FUBP1, ENO1^b, LGALS3^b
Cell signaling	2.191–1.325	HSPA5, STMN1^b, NP^b, PRDX^b, THBS1, NME1^b, HSPB1, PPIA^b
Antigen presentation	2.156–1.420	LGALS3^b, ANXA1^b, THBS1, ANXA2^b, HSP90B1

The significance value associated with Functional Analysis for a dataset is a measure of the likelihood that the association between a set of molecules and a given process or pathway is due to random chance. The smaller the P value the less likely that the association is random and the more significant the association is. In general, P values less than 0.05 [-log (P) > 1.3] indicates statistically significant, nonrandom association. The P value is calculated using the right-tailed Fisher Exact test.

Proteins that nitrosylation was significantly altered by E2.