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. 2010 Jun 9;151(8):3918–3928. doi: 10.1210/en.2010-0239

Figure 3.

Figure 3

Tissue distribution of guinea pig Prl and Gh mRNAs. A, Northern blot analysis for Prl mRNA in the pituitary gland and uterus of a cycling female guinea pig (NP) and in the uterus, placenta, and subplacenta from gestation d 35 pregnant guinea pigs. B, RT-PCR analysis for Prl mRNA in the pituitary gland and uterus of a cycling female guinea pig (NP) and in the uterus, pituitary, placenta, and subplacenta of gestation d 35 pregnant guinea pigs. C, Northern blot analysis for Prl mRNA in pituitary, ovary, mammary gland, testis, heart, lung, liver, kidney, brain, and muscle of gestation d 35 pregnant guinea pigs. D, RT-PCR analysis for Prl mRNA in the same tissues shown in C. E, Northern blot analysis for Gh mRNA in pituitary gland, mammary gland, heart, lung, liver, kidney, brain, and muscle. F, RT-PCR analysis for Gh mRNA in pituitary gland, ovary, mammary gland, testis, heart, lung, liver, kidney, brain, muscle, uterus, and placenta. Expression of 18S RNA was used as an internal control for RNA integrity. Note that guinea pig Prl and Gh mRNAs were exclusively expressed in pituitary gland.