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. 2010 Jun 9;151(8):3577–3588. doi: 10.1210/en.2010-0233

Figure 6.

Figure 6

CCK promotes β-cell survival. A, MIN-6 cells were reverse transfected with si-Scr negative control or si-Cck oligonucleotides (n = 3). MIN-6 were then incubated for 48 h before being treated for 24 h with IL-1β and TNF-α cytokine cocktail and cell death measured. Comparisons were made by repeated-measures ANOVA followed by Bonferroni-corrected Student’s paired t test. B, Isolated islets from CckWT-ob/ob (n = 9) and CcklacZ-ob/ob (n = 17) mice were dispersed and treated with 10 μm thapsigargin. Islet cells were incubated for 24 h, and cell death was measured. Comparisons were made by repeated-measures ANOVA followed by Bonferroni-corrected Student’s paired t test. C, Islets from CcklacZ-ob/ob (n = 8–11) mice were isolated, dispersed, and treated with 10 μm thapsigargin in the presence of increasing doses of sulfated CCK-8 peptide or vehicle control. Data were analyzed by ANOVA blocking on sample (P < 0.005). Using the vehicle as a covariate, CCK dose followed a log-linear trend to reduce islet cell death (P < 0.05).