Table 1.
TaqMan real-time PCR primers and probes
| Gene name | Primer sequence (5′–3′) | Probe sequence (5′–3′) | GenBank accession ID |
|---|---|---|---|
| KiSS-1 | 6FAM-CAGGCCAGCAGCTGGAATCCCTG-TAMRA | AY823262 | |
| Forward | AGAAAAGGTGGCCTCTGTGGA | ||
| Reverse | AGGCTCTGCTCCCACGG | ||
| NKB | 6FAM-ACAGCAAGAGGGATCT-MGB | XM_001115535 | |
| Forward | GAGCCACAGGAGGAGATGGTT | ||
| Reverse | TCCAGAGATGAGTGGCTTTTGA | ||
| PDYN | 6FAM-CCAGTTCAAGGTGGTGAC-MGB | XM_001113720 | |
| Forward | CAAGCTCAAGTGGGACAACCA | ||
| Reverse | CTCCAGAGTAAGCATTCGGATCTT | ||
| GPR54 | 6FAM-ACAAGCCGATGCGGACCGTGA-TAMRA | AY823261 | |
| Forward | AACTCGCTGGTCATCTACGTCAT | ||
| Reverse | TCTGTGGCCGCCAGGTT | ||
| NK3 | 6FAM-ATGGCTATTATTGATCCCCTGAAACCCAGA-MGB | AB180075 | |
| Forward | CCATTGCGGTGGACAGGTA | ||
| Reverse | TTGGTTGCTGTGGCAGACA | ||
| KOR | 6FAM-TGGTGGAGGCTCTG-MGB | XR_012897 | |
| Forward | GCTGGACCCCCATTCACATA | ||
| Reverse | GCCTAAGGCGATGCAGAAGT | ||
| ALG9 | 6FAM-ACTGTCTTCCTGTTCGGG-MGB | XM_001106180 | |
| Forward | AACAGTGCCACAGAGCGAGAA | ||
| Reverse | CGATACCGCCTGGAGCACTA | ||
| RPL13A | 6FAM-CCAGGCAGTGACAGCCACCTTGG-MGB | XM_001115079 | |
| Forward | TCACGAGGTTGGCTGGAAGT | ||
| Reverse | GATCTTGGCTTTCTCCTTCCTCTT |
Reference sequences for each of the targeted Macaca mulatta genes can be accessed via GenBank accession ID.