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. 2010 Jun 9;151(8):3589–3599. doi: 10.1210/en.2010-0106

Figure 6.

Figure 6

Leptin potentiates CCK-induced CART abundance in vagal afferent neurons. Neurons were cultured for 72 h and stained with antibody to CART. A, CART fluorescence in cells with a fluorescent intensity more than 2 sd above control were quantified. Neither 1 nm CCK8s nor 10 ng/ml leptin alone had an effect on the abundance of CART immunoreactivity compared with unstimulated cells; however, there was a significant increase in CART abundance when vagal afferent neurons were stimulated with both 1 nm CCK8s and 10 ng/ml leptin. The response to 10 nm CCK8s was further increased by leptin. B, The percentage of cells exhibiting fluorescent intensity more than 2 sd above control was increased to comparable proportions when stimulated with 10 nm CCK8s, 1 nm CCK8s, and 10 ng/ml leptin, or 10 nm CCK8s and 10 ng/ml leptin, whereas no significant increase was observed with either 1 nm CCK8s or 10 ng/ml leptin alone compared with unstimulated cells (n = 3).