Figure 2.

Hepatic IR and IRS expression in fed and fasting states. Male CD1 mice (10 wk old) were fasted for 16 h (n = 6) or fed ad libitum (n = 6). Liver tissue was collected from killed mice for the preparation of liver protein extracts. Aliquots of 20-μg liver proteins were subjected to immunoblot assay. Hepatic IR (A), IRS1 (B), and IRS2 (C) protein levels were determined. Additional aliquots of liver tissue (20 mg) were homogenized for the preparation of nuclear and cytoplasmic fractions, followed by the determination of FoxO1 in the nucleus (D) and cytoplasm (E) of hepatocytes in fed and fasted mice. *, P < 0.05 vs. control. NS, Not significant.